Loading and Running DNA Samples on an Agarose Gel: Step-by-Step Guide

🔬Agarose gel electrophoresis is a technique used to separate DNA fragments based on their size.

💡DNA fragments move from the negative electrode to the positive electrode due to their negative charge.

🌡️The mini-sub cell has an electrode wire that allows the electric current to pass through the gel, separating the DNA fragments.

🧪The agarose gel is placed in a gel chamber and submersed in electrophoresis running buffer.

⚡Applying constant voltage to the gel chamber starts the flow of current and separates the DNA fragments.

What is agarose gel electrophoresis used for?

—Agarose gel electrophoresis is primarily used to separate DNA fragments based on their size.

What determines the direction of DNA movement in the gel?

—DNA fragments move from the negative electrode to the positive electrode due to their negative charge.

What is the purpose of the mini-sub cell in agarose gel electrophoresis?

—The mini-sub cell has an electrode wire that allows the electric current to pass through the gel, separating the DNA fragments.

What is added to the gel chamber during agarose gel electrophoresis?

—Electrophoresis running buffer is added to the gel chamber to provide the necessary environment for DNA separation.

How does agarose gel electrophoresis separate DNA fragments?

—Applying constant voltage to the gel chamber creates an electric field that causes the DNA fragments to move and separate based on their size.

00:06The video is a step-by-step guide on loading and running DNA samples on an agarose gel.

00:16A mini-sub cell is used for agarose gel electrophoresis.

00:27The electrode wire in the mini-sub cell allows electric current to pass through the gel, separating the DNA fragments.

00:32Position the gels so that the wells are closest to the negative electrode.

00:56Add electrophoresis running buffer to the gel chamber and ensure the wells are covered.

01:17Load the DNA samples in the correct order according to the assigned lanes.

01:28Use an adjustable micropipette to slowly transfer the DNA samples into the wells.

02:08Apply pressure to the plunger button to fill the wells with the DNA samples.